Rotating Disc Shear Device
The kompAs ultra scale-down (USD) shear device is designed to produce controlled levels of energy dissipation similar in range to those found in industrial scale processing equipment, such as centrifuges, pumps and mixers. A particular application is the mimic of the hydrodynamic shear forces encountered in the feed zone of high-speed, continuous flow, industrial-scale centrifuges. By exposing process material to shear forces in this way, it it possible to:
- determine the susceptibility of the material of interest (e.g. cells, proteins, macromolecules, etc.) to damage caused by processing
- condition or prepare the process material to closely resemble that found at industrial scale for further processing studies (e.g. USD centrifugation or filtration)
kompAs comprises a sample processing unit and a speed control unit. The sample processing unit consists of a hollow chamber (20 mL internal capacity) inside which a rotating disc is fitted. Both are made of stainless steel. The speed of the rotating disc (0-18,000 rpm) is adjusted via the controller. The device sample chamber is fitted with a double envelope so that the temperature control can be achieved through circulation of cooling/heating liquid. The choice of the disc speed and time (max. 1 min) of sample exposure to shear is dependent on the specific process operation to be mimicked.
- Enables early stage manufacturability assessment
- Provides insight on manufacturability
- Mimics larger-scale environment. The device speeds are equivalent to energy dissipation rates of 103 to106 W kg-1 and shear rates in the order of 104 to 106 s-1 which correspond to the hydrodynamic conditions in most bioprocess equipment
- Small sample volume. Large-scale manufacturing insights using sample volume of just 20 mL
- Rank process materials. Identify which process materials have robust product forms, increased impurities, or aggregate formation after exposure to shear stress
- Link with other scale-down tools. Generate process materials for other scale-down tools, for example the USD centrifugation, to investigate how exposure to shear stress impacts processing operations further downstream
- Examine impact of shear stress without air-liquid interfaces. Designed to remove air bubbles to ensure that the energy dissipation is only due to hydrodynamic shear stress and not due to air-liquid interfaces.
USD studies of the effect of shear on crystals, flocs, genetically modified plants, human cells for therapy, mammalian cells, precipitates, proteins and enzymes
For further information or for a quote, please contact us.